The experimental results are determined by plotting the concentration of the standard solutions on the x-axis and the corresponding OD (optical density) values on the y-axis. A standard curve is then drawn either manually on graph paper or using software. Once the curve is established, the OD value obtained from the sample is used to find the corresponding concentration on the curve. Alternatively, a linear regression equation is derived from the standard curve data, which relates the concentration of the standards to their OD readings. The OD value of the sample is then plugged into this equation to calculate its actual protein S concentration. This method ensures accurate quantification by relying on the linear relationship between concentration and OD, allowing for precise determination of the target protein in the sample. The process is widely used in ELISA assays due to its reliability and ease of interpretation. It is important to ensure that the samples fall within the linear range of the standard curve to avoid inaccurate results. Proper calibration and careful handling of reagents also play a key role in achieving consistent and reproducible outcomes.
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