The experimental results are determined by plotting the standard concentrations on the x-axis and the corresponding OD values on the y-axis. A standard curve is then created on graph paper or using software. By analyzing the OD value of the sample, its corresponding concentration can be estimated from the standard curve. Alternatively, a linear regression equation is derived from the relationship between the standard concentrations and their OD readings. The OD value of the sample is then input into this equation to calculate its actual concentration. This method ensures accurate and reliable quantification of Human Protein S in the tested samples. It is essential to follow proper calibration procedures and ensure that all reagents are handled correctly to maintain the integrity of the results. Additionally, it's recommended to run duplicate or triplicate samples to improve the precision of the measurement. Proper documentation of each step helps in tracking any potential errors and allows for reproducibility of the experiment.
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