PCR gene amplification instrument three basic reaction steps

Shanghai Xinzhuang Instrument Co., Ltd. Xinzhuang (LNB) PCR is a large-scale synthesis of In Vitro in vitro or in vitro using DNA polymerase. Basically, it uses DNA polymerase for specific linkage replication. At present, the commonly used technology can copy a gene into the original 10 billion to 100 billion times. According to the purpose of DNA amplification and the standards of detection, the PCR instrument can be divided into four types: ordinary PCR instrument, gradient PCR instrument, in situ PCR instrument, and real-time fluorescence quantitative PCR instrument.

How to use the gene amplification PCR instrument:

PCR consists of three basic reaction steps: denaturation-annealing (refolding)--extension: 1 denaturation of template DNA: template DNA is heated to 90-95 ° C for a certain period of time, then the template DNA is double-stranded or amplified by PCR. The double-stranded DNA dissociates into a single strand so that it binds to the primer to prepare for the next round of reaction; 2 anneals (refolds) of the template DNA and the primer: the template DNA is denatured into a single strand by heating, and the temperature is lowered. To 55~60 °C, the primers are paired with the complementary sequence of the single strand of the template DNA; 3 primer extension: DNA template--primer conjugate under the action of DNA polymerase, at 70-75 ° C, using dNTP as the reaction material, The target sequence is a template, and a new semi-reserved replication chain repeating cyclic denaturation-annealing-extension process is synthesized according to the principle of base pairing and semi-reserved replication. The copy chain, and this new chain can be the template for the next cycle. It takes 2 to 4 minutes to complete each cycle, and the amplification of the gene to be amplified can be amplified several million times in 2 to 3 hours.

Shanghai Xinzhuang Instrument Co., Ltd. PCR instrument, fast temperature rise, accurate temperature uniformity, there are a variety of models, please contact us!

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