Human Lymphocyte Antigen 6 Complex Locus A (Ly6a) ELISA Kit, Alias: Human Lymphocyte Antigen 6 Complex Locus A (Ly6a) Assay Kit, Human Lymphocyte Antigen 6 Complex Locus A (Ly6a) Immunosorbent assay kit.
English name: humanlymphocyteantigen6complexlocusA, Ly6aELISAKit
Specification: 48T/96T
Use (for scientific research only, not for clinical use):
It is used to determine the content or activity of human lymphocyte antigen 6 complex locus A (Ly6a) in serum, plasma and related liquid samples.
The company provides ELISA test services:
The purchasing ELISA kit provides free testing services. You only need to send the specimens. We will save you time and help you with the results. The raw data and analysis data can be provided. The experiment time is usually about one week to give you the results.
We have professional technicians to provide you with technical guidance throughout the process. Including pre-sale specimen collection, unclear use during the process, data analysis after the end of the experiment, we will communicate with you immediately if you have any questions.
There may be some problems during the elisa experiment. The following is a brief summary of the common problems and solutions for the elisa experiment. I hope I can help you.
Problem: The background is deep, all are colored, and the critical control OD value is out of the specified range.
The possible reasons for this problem are as follows:
1. Insufficient washing, no dryness after washing, residue of other components in the sample or residue of enzyme label
Solution: Accurate preparation of concentrated washing solution; if there is crystallization of 10 times concentrated washing solution, the crystal should be dissolved at room temperature and then diluted; fully wash and thoroughly pat dry. The filter paper for adding or adding the enzyme plate should be discarded and not used repeatedly, otherwise it will cause pollution.
2, sample contamination
Solution: Samples should be freshly collected or stored at low temperatures to prevent contamination.
3, the incubator temperature exceeds 37 ° C or the reaction time is too long
Solution: Adjust the temperature of the incubator and accurately time it.
4, the nozzle is reused, not washed or disinfected thoroughly
Solution: Use the nozzle as soon as possible.
5. Distilled water is contaminated
Solution: Use fresh distilled water.
6, enzymes and other reagents mixed
Solution: Do not mix different batch reagents.
7. The amount of specimens in one experiment is too much, and the loading time is too long, resulting in an extended reaction time.
Solution: Arrange the experiment reasonably and avoid several samples of the enzyme plate at the same time.
Problem: poor repeatability
The possible reasons for this problem are as follows:
1. The number of samples varies, and the loading time is long and short.
Solution: When repeating a sample, the loading time is as close as possible to the first time.
2, the holding time is inconsistent, the washing conditions are inconsistent
Solution: Repeat the test specimens, operating conditions, personnel, etc. should be as consistent as possible with the previous time to eliminate the possibility of inconsistency caused by these factors.
3, the amount of sample is inconsistent
Solution: Mix the sample thoroughly before diluting, use the same pipette as much as possible and tighten the nozzle.
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