Human Chikungunya virus (CHIKV) ELISA test kit

The Chikungunya Virus (CHIKV) ELISA Kit is designed for the quantitative detection of CHIKV in human biological samples using a double-antibody one-step sandwich ELISA method. The process begins with pre-coated microplates containing specific capture antigens for CHIKV. Following incubation with standards, samples, and HRP-conjugated detection antibodies, the plate undergoes washing to remove unbound components. A TMB substrate is then added, which changes color under the action of peroxidase—first turning blue and then yellow when an acidic stop solution is introduced. The intensity of the color correlates directly with the concentration of CHIKV in the sample. The absorbance is measured at 450 nm using a microplate reader, allowing for accurate quantification. **Sample Collection and Preparation:** 1. **Serum:** Collect blood in pyrogen-free tubes, centrifuge at 3000 rpm for 10 minutes to separate serum from red blood cells. 2. **Plasma:** Use anticoagulants like EDTA, citrate, or heparin, then centrifuge at 3000 rpm for 30 minutes to obtain plasma. 3. **Cell Supernatant:** Centrifuge at 3000 rpm for 10 minutes to remove debris. 4. **Tissue Homogenate:** Homogenize tissue in physiological saline, then centrifuge at 3000 rpm for 10 minutes to collect supernatant. 5. **Storage:** Store samples at -20°C in single-use aliquots. Avoid repeated freezing and thawing. Thaw at room temperature before use. **Required Equipment:** - Microplate reader (450 nm) - Precision pipettes (0.5–10 µL, 2–20 µL, 20–200 µL, 200–1000 µL) - Incubator set at 37°C **Precautions:** - Store the kit at 2–8°C. Allow it to equilibrate at room temperature for 20 minutes before use. - Reconstituted washing buffer may crystallize; gently warm it in a water bath before use. - Unused wells should be returned to the sealed bag immediately. - No dilution is required for pre-treated samples; add 10 µL directly. - Follow the incubation time and sequence precisely. - Shake all reagents thoroughly before use. **Kit Components (96-well format):** - Microwells: 12 × 8 - Standards (1000 pg/mL): 0.5 mL - Standard Dilutions: 6 mL - Sample Diluent: 6 mL - Detection Antibody-HRP: 6 mL - 20× Wash Buffer: 20 mL - Substrate A & B: 6 mL each - Stop Solution: 6 mL - Seal Film: 2 sheets - Ziplock Bag: 1 **Reagent Preparation:** - Dilute 20× Wash Buffer by mixing 1 part with 19 parts of distilled water. **Washing Procedure:** - Manual: Wash 5 times with wash buffer, letting each well stand for 1 minute. - Automatic: Inject 350 µL of wash buffer per well, soak for 1 minute, repeat 5 times. **Procedure Steps:** 1. Remove the required microplate strips from the foil pouch after 20-minute room temperature equilibration. 2. Set up standard, sample, and blank wells. Add 50 µL of standards and 10 µL of sample plus 40 µL diluent. 3. Add 50 µL of HRP-labeled antibody to each well. Seal and incubate at 37°C for 60 minutes. 4. Wash 5 times as described. 5. Add 50 µL of TMB A and B, incubate in the dark for 15 minutes. 6. Add 50 µL of stop solution and measure OD at 450 nm within 15 minutes. **Data Analysis:** - Plot standard concentrations vs. OD values in Excel to generate a standard curve. - Use the curve equation to calculate sample concentrations. **Kit Performance:** - Accuracy: R ≥ 0.9900 - Sensitivity: <1.0 pg/mL - Specificity: No cross-reactivity with other viruses - Reproducibility: CV <15% - Storage: 2–8°C, protected from light and moisture - Shelf Life: 6 months - Detection Range: 31.2 pg/mL – 1000 pg/mL **Disclaimer:** - This kit is for research purposes only. Not suitable for clinical use. - The manufacturer is not responsible for misuse or deviation from instructions. - Do not mix reagents from different batches. All procedures must be followed strictly.

Poly L-lactic Acid PLLA

Poly L-lactic acid (PLLA) is a biodegradable, biocompatible, and synthetic polymer derived from lactic acid. Due to its excellent safety profile and natural bioresorption, PLLA is widely used in medical, pharmaceutical, and industrial applications, including aesthetic treatments, tissue engineering, sutures, and drug delivery systems.

The CAS Registry Number for Poly(L-lactic acid) (PLLA) is 33135-50-1. It is available as a white powder, with the following specifications:

1. Intrinsic Viscosity: Ranges from 0.30 to 2.25 dl/g

2. Molecular Weight (Mw): Available from 6,000 to 30,000

Each batch undergoes strict quality control, and a Certificate of Analysis (CoA) is provided, detailing the analytical data from our laboratory. Additional analytical data can be supplied upon request to meet specific requirements.

For optimal stability, PLLA should be stored in its original packaging at low temperatures (2-8°C). Under these conditions, it retains its initial properties for at least two years.

We also suppy medical grade PCL/PLGA/PDLA/PDLLA.

Contact us for more details or to discuss customized specifications based on your needs!

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