Human Chikungunya virus (CHIKV) ELISA test kit

The Chikungunya virus (CHIKV) ELISA Kit is designed for the quantitative detection of CHIKV in human biological samples using a sandwich ELISA method. This technique involves pre-coated microwells with specific capture antigens for CHIKV. Following incubation with the sample, standard, and HRP-labeled detection antibodies, the plate is washed thoroughly to remove unbound components. A TMB substrate is then added, which changes color under the action of peroxidase. The reaction is stopped with an acidic solution, resulting in a yellow color whose intensity is directly proportional to the concentration of CHIKV in the sample. The optical density (OD) is measured at 450 nm using a microplate reader, allowing for accurate quantification. **Sample Collection and Preparation** 1. **Serum**: Collect blood in endotoxin-free tubes, centrifuge at 3000 rpm for 10 minutes, and carefully separate the serum from red blood cells. 2. **Plasma**: Use anticoagulants such as EDTA, citrate, or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant. 3. **Cell Supernatant**: Centrifuge at 3000 rpm for 10 minutes to remove debris before collecting the supernatant. 4. **Tissue Homogenate**: Homogenize the tissue in physiological saline, then centrifuge at 3000 rpm for 10 minutes to obtain the supernatant. 5. **Storage**: If not used immediately, aliquot the sample and store at -20°C. Avoid repeated freeze-thaw cycles. Thaw at room temperature and ensure complete thawing before testing. **Required Equipment** - Microplate reader (450 nm) - High-precision pipettes: 0.5–10 μL, 2–20 μL, 20–200 μL, 200–1000 μL - Incubator set at 37°C **Precautions** - Store the kit at 2–8°C. Allow it to equilibrate at room temperature for 20 minutes before use. - Any unused wells should be returned to the ziplock bag and stored properly. - Do not dilute the pre-treated samples; add 10 μL directly. - Follow the incubation time and sequence strictly as outlined in the instructions. - Ensure all reagents are well mixed before use. **Kit Components** - Microwell plates (96-well or 48-well configuration) - Standard (1000 pg/ml) - Diluted standard solutions (various concentrations) - Sample diluent - Detection antibody-HRP - 20× Wash buffer - Substrate A and B - Stop solution - Seal film - Ziplock bag **Reagent Preparation** Dilute the 20× wash buffer by mixing 1 part with 19 parts of distilled water. **Washing Procedure** - Manual: Add washing solution, let stand for 1 minute, drain, and repeat 5 times. - Automatic: Inject 350 μL of washing solution per well, soak for 1 minute, and repeat 5 times. **Procedure** 1. Remove the required number of wells from the pouch after 20 minutes of equilibration. 2. Set up standard, sample, and blank wells. 3. Add 50 μL of standards and 10 μL of sample plus 40 μL of diluent. 4. Add 50 μL of HRP-labeled antibody to each well, seal, and incubate at 37°C for 60 minutes. 5. Wash the plate 5 times. 6. Add 50 μL of TMB substrate A and B, incubate in the dark for 15 minutes. 7. Stop the reaction with stop solution and measure OD at 450 nm within 15 minutes. **Data Analysis** Plot the standard curve using Excel, with concentration on the x-axis and OD on the y-axis. Calculate the sample concentration based on the regression equation. **Kit Performance** - Accuracy: R value ≥ 0.9900 - Sensitivity: <1.0 pg/mL - Specificity: No cross-reactivity with other similar antigens - Repeatability: CV <15% between plates - Storage: 2–8°C, protected from light and moisture - Shelf Life: 6 months - Detection Range: 31.2 pg/mL – 1000 pg/mL **Disclaimer** This kit is intended for research purposes only. It must not be used in clinical trials or human experiments. The company is not responsible for any misuse or deviation from the instructions. Always follow the protocol carefully. Do not mix different batch numbers. The user assumes full responsibility for any errors or consequences.

Beauty And Personal Care Products

Beauty and Personal Care Products with REBORN Collagen Stimulator

As we age, the fat, muscles, bone, and skin in our face begins to thin. This loss of volume leads to either a sunken or sagging appearance of the face and skin. Injectable poly-l-lactic acid filler is used to create structure, framework, and volume to the face. Aging, environmental stress, and lifestyle factors can take a toll on your skin, leading to fine lines, wrinkles, volume loss, and reduced elasticity. While traditional skincare can offer temporary hydration, Reborn PLLA Dermal Filler provides a long-lasting, non-surgical solution to rejuvenate and restore skin from within.

What is REBORN PLLA Dermal Filler?

Reborn PLLA (Poly-L-Lactic Acid) Dermal Filler is an advanced collagen stimulator designed to naturally restore skin volume, improve elasticity, and reduce signs of aging. Unlike traditional HA fillers, which provide instant but temporary results, PLLA fillers works gradually by stimulating the body’s natural collagen production, ensuring a more youthful and long-lasting transformation.

PLLA Filler or Reborn collagen stimulator, as injectable poly-l-lactic acid(PLLA) is used to create structure, framework, and volume to the face. PLLA(poly lactic acid) is known as a bio-stimulator dermal filler, this means it stimulates your own skin to produce new collagen. Over time your skin breaks down PLLA filler into water and carbon dioxide.

Body Care,Eyes Care,Face Care,Hands And Feet Care

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