Human Melanocyte Antibody (MC Ab) ELISA Kit Instruction Manual Elisa kit Specification: 48-well configuration / 96-well configuration Standard dilution: 1.5ml × 1 bottle of enzyme label reagent: 3 ml × 1 bottle (48) / 6 ml × 1 bottle (96) [Human Melanocyte Antibody (MC Ab) ELISA Kit] This reagent is for research use only: the standard concentration is the abscissa, the OD value is the ordinate, and the standard curve is drawn on the coordinate paper. Find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or calculate the linear regression equation of the standard curve using the concentration of the standard and the OD value, and substitute the OD value of the sample into the equation to calculate the sample concentration , Multiplied by the dilution factor, which is the actual concentration of the sample. Kit composition: sealing film: 2 pieces (48) / 2 pieces (96) Instructions: 1 sealed bag: 1 standard product: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle at 2-8 ℃ to store enzyme Standard coated plate: 1 × 48 1 × 96 2-8 ° C Store the sample diluent: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Store the developer A solution: 3ml × 1 bottle 6 ml × 1 bottle 2 -8 ℃ storage of developer B solution: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ℃ storage stop solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ℃ storage concentrated washing solution: (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle stored at 2-8 ℃. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human melanocyte antibody (MC Ab) in the specimen. The microplate was coated with purified human melanocyte antibody (MC Ab) antibody to make a solid-phase antibody, and anti-platelet antibody IgG / M / A (PA-IgG / M / A was added to the microwells coated with mAb in turn ), And then combined with HRP-labeled anti-platelet antibody IgG / M / A (PA-IgG / M / A) antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the anti-platelet antibody IgG / M / A (PA-IgG / M / A) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human melanocyte antibody (MC Ab) in the sample was calculated by a standard curve. Purpose: This kit is used to measure the content of anti-platelet antibody IgG / M / A (PA-IgG / M / A) in human serum, plasma and related liquid samples.
Hard alloy Lianxiang tungsten steel for milling cutter bit
Hard alloy Lianxiang tungsten steel for milling cutter bit
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